diff --git a/chrome/browser/extensions/api/tabs/tabs_api.cc b/chrome/browser/extensions/api/tabs/tabs_api.cc
index 0801bd6cb804a..7884b0ee3767a 100644
--- a/chrome/browser/extensions/api/tabs/tabs_api.cc
+++ b/chrome/browser/extensions/api/tabs/tabs_api.cc
@@ -468,6 +468,18 @@ int MoveTabToWindow(ExtensionFunction* function,
     return -1;
   }
 
+#if !BUILDFLAG(IS_ANDROID)
+  // Match the guard in WindowsCreateFunction::ValidateTab(): an Isolated Web
+  // App's tab must never be detached from its dedicated app window.
+  if (Browser* src = source_window->GetBrowser()) {
+    if (web_app::AppBrowserController* controller = src->app_controller();
+        controller && controller->IsIsolatedWebApp()) {
+      *error = kWindowCreateCannotMoveIwaTabError;
+      return -1;
+    }
+  }
+#endif
+
   TabListInterface* source_tab_list = TabListInterface::From(source_browser);
   ::tabs::TabInterface* tab = source_tab_list->GetTab(source_index);
   if (!tab) {