diff --git a/chrome/browser/media/webrtc/display_media_access_handler.cc b/chrome/browser/media/webrtc/display_media_access_handler.cc
index e01688c08958c..4dff3d175a80d 100644
--- a/chrome/browser/media/webrtc/display_media_access_handler.cc
+++ b/chrome/browser/media/webrtc/display_media_access_handler.cc
@@ -456,6 +456,23 @@ void DisplayMediaAccessHandler::ProcessQueuedAccessRequest(
   const PendingAccessRequest& pending_request = *queue.front();
   UpdateTrusted(pending_request.request, false /* is_trusted */);
 
+  // Re-check that the requesting frame is still active. The request was
+  // validated at enqueue time in HandleRequest(), but the primary main frame
+  // may have navigated cross-origin while this request was queued behind
+  // another picker. Showing the picker now would attribute it (via
+  // GetApplicationTitle(web_contents)) to the post-navigation origin.
+  {
+    RenderFrameHost* const rfh =
+        RenderFrameHost::FromID(pending_request.request.render_process_id,
+                                pending_request.request.render_frame_id);
+    if (!rfh || !rfh->IsActive()) {
+      RejectRequest(
+          web_contents,
+          MediaStreamRequestResult::FAILED_DUE_TO_SHUTDOWN_NO_RFH_IN_HANDLER);
+      return;
+    }
+  }
+
   const GURL& request_origin = pending_request.request.security_origin;
   AllowedScreenCaptureLevel capture_level =
       capture_policy::GetAllowedCaptureLevel(request_origin, web_contents);